Iplate yeNkcubeko
| E21.2310 | ||||
| ORE HAYI. | Umzekelo | I-QTY / CTN | GW / CTN | Ubukhulu |
| 2057 | 96hole U ukumila | Ii-100pcs | 8kg | 28 * 28 * 25cm |
| 2058 | I-96hole U imilo yomgca we-12 | Ii-100pcs | 8kg | 28 * 28 * 25cm |
| 2059 | Imilo engama-96 V | Ii-100pcs | 8kg | 28 * 28 * 25cm |
| 2061 | I-96hole iflethi iphantsi | Ii-100pcs | 8kg | 28 * 28 * 25cm |
| 2062 | I-96hole iflethi iphantsi* 12 umgca | Ii-100pcs | 8kg | 28 * 28 * 25cm |
Iplate yenkcubeko yeseli inokwahlulwa ibe yiplati esezantsi kunye nezantsi ejikelezileyo (emile ngoku-U kunye ne-V) ngokwimo yezantsi; inani lemithombo yenkcubeko yi-6, 12, 24, 48, 96, 384, 1536, njl. kuxhomekeka kwimathiriyeli, kukho ipleyiti yeTerasaki kunye neplate yesiqhelo yenkcubeko yeseli. Ukhetho oluthile luxhomekeke kuhlobo lweeseli ezikhulisiweyo, ivolumu yenkcubeko efunekayo kunye neenjongo ezahlukeneyo zovavanyo.
(1) Umahluko kunye nokukhetha amacwecwe enkcubeko athe tyaba-ezantsi kwaye ajikeleze ezantsi (ezimilise u-V kunye ne-V)
Iimo ezahlukeneyo zamacwecwe enkcubeko zisetyenziswa ngeendlela ezahlukeneyo. Ukulungiselela iiseli zokuhlaziya, indawo esezantsi ethe tyaba ihlala isetyenziswa, ekulungeleyo ukujongwa phantsi kwemicroscope, inendawo ecacileyo esezantsi, kwaye ukuphakama kwenqanaba lolwelo lwenkcubeko yeseli kuyahambelana. Ke ngoko, xa usenza iimvavanyo ezinje nge-MTT, nokuba ziyabambelela okanye iiseli ezinqunyanyisiweyo, isitya esisezantsi esisicaba sisetyenziswa ngokubanzi. Ipleyiti yenkcubeko ethe tyaba-emazantsi kufuneka isetyenziselwe ukulinganisa ixabiso lokufunxeka. Nika ingqwalasela ekhethekileyo kwizinto eziphathekayo. Ilebheli "yeTissue Culture (TC) iphathwe" yenzelwe inkcubeko yeseli. Iipleyiti ezimile okwe-U okanye ezimilise u-V zihlala zisetyenziswa xa kufuneka iimfuno ezizodwa. Umzekelo, kwi-immunology, xa ii-lymphocyte ezimbini ezahlukeneyo zixubene nenkcubeko, ezi zimbini kufuneka zinxibelelane ukuze zikhuthaze. Kule meko, iipleyiti ezimile okwe-U zihlala zisetyenziswa kuba iiseli ziya kuqokelelana kwindawo encinci ngenxa yomxhuzulane. Ipleyiti yenkcubeko ejonge ezantsi nayo iya kusetyenziswa kulingo lokufakwa kwesotope, kwaye kuyimfuneko ukusebenzisa isivuni sesisele ukuqokelela iiseli zenkcubeko, ezinje "ngenkcubeko ye-lymphocyte exubekileyo" njalo njalo. Iipleyiti ezimile ngoku-V zihlala zisetyenziselwa ukubulala iseli kunye nokuvavanywa kweemagnaglutination ze-immunological. Isilingo sokubulala iseli sinokutshintshwa yipleyiti emise okwe-U (emva kokudibanisa iiseli, icentrifugation ngesantya esisezantsi).
(2) Umahluko phakathi kweplate yeTerasaki kunye neplate yesiko eliqhelekileyo lenkcubeko
Isitya seTerasaki sisetyenziselwa uphando lwe-crystallographic, kwaye uyilo lwemveliso lukulungele ukujongwa kwekristal kunye nohlalutyo lolwakhiwo. Zimbini iindlela zokuhlala kunye nokunikezela ngokudropha, kwaye ukubonakala kunye nolwakhiwo lweemveliso zesicelo kwezi ndlela zimbini zahlukile. Khetha ikristale yekristale yekristale njengezinto, kwaye izinto ezizodwa zilungile ekuqwalaseleni ulwakhiwo lwekristale. Ipleyiti yenkcubeko yeseli ikakhulu yenziwe ngezinto zePS, kwaye izinto ziphathwa ngokwaneleyo, ezilungele ukukhula kweseli kunye nokwandiswa. Ewe kunjalo, kukho izinto zokukhula zeeseli zeplanktonic, kunye nomgangatho ophantsi wokubopha.
(3) Umahluko phakathi kwepleyiti yenkcubeko yeseli kunye nemicroplate
Iipleyiti ezinelebhile ye-enzyme zihlala zibiza kakhulu kunepleyiti yenkcubeko yeseli. Iipleyiti zeseli zisetyenziselwa inkcubeko yeseli kwaye zinokusetyenziselwa ukulinganisa uxinaniso lweprotein. Iipleyiti ezinelebhile ye-enzyme zibandakanya iipleyiti zokwaleka kunye neepleyiti zokuphendula. Ngokubanzi, akukho mfuneko yokuba isetyenziselwe inkcubeko yeseli. Zisetyenziselwa ukuphendula ngokungafunekiyo kwe-immunoenzyme. Ukufunyanwa kweprotein kamva kufuna iimfuno eziphezulu kunye nolwelo oluthile olubhalwe i-enzyme.
(4) Ummandla ongezantsi wemithombo yamacwecwe enkcubeko ahlukeneyo kunye nenani elicetyiswayo lolwelo akufuneki libe nzulu kakhulu, ngokubanzi kuluhlu lwe-2 ~ 3mm, kwaye indawo engezantsi yemithombo eyahlukeneyo ingasetyenziselwa ukubala iqula ngalinye. Ixabiso elifanelekileyo lolwelo eliza kongezwa (jonga kwitheyibhile engezantsi). Ukuba inani lolwelo elongeziweyo lininzi kakhulu, liyakuchaphazela ukutshintshiselana ngegesi (ioksijini), kwaye kulula ukuphuphuma kwaye kubangele ungcoliseko ngexesha lenkqubo yokuhamba. Ubuninzi beeseli ezongeziweyo ziya kuxhomekeka kwinjongo yovavanyo ngokuguqukayo.
